Thioredoxin fusion expression vector
WebThe pET-32 vector series is designed for high-level expression of recombinant thioredoxin fusion proteins. Previous studies have shown that linkage to thioredoxin increases the … WebThe pBAD-DEST49 vector features: • The araBAD promoter for tightly regulated expression in E. coli • HP-thioredoxin fusion for improved protein yield and solubility • Enterokinase …
Thioredoxin fusion expression vector
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WebFeb 8, 2024 · Abstract. In this paper, we report a soluble expression based on Escherichia coli and two-step purification of a novel thioredoxin-tagged chicken interferon-α fusion protein (Trx-rChIFN-α) by using pET32a(+) expression system. The mature ChIFN-α gene was amplified by Reverse transcriptase-polymerase chain reaction (RT-PCR) and … WebFeb 1, 1993 · Abstract. We have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the production of a variety of mammalian cytokines and growth factors as thioredoxin fusion proteins. Although many of these cytokines previously ...
WebFeb 1, 2012 · In one previous approach, LL-37 coding sequence was cloned into vector pET-32a, allowing the peptide to be expressed as a thioredoxin fusion. The fusion protein contains two thrombin cleavage sites: a vector-encoded one that is 30-residue upstream of the insert and an engineered one that is immediately adjacent to LL-37. WebAbaecin, a 34-aa-long antimicrobial peptide from honeybees, was expressed in a fusion form to 6xHis-SUMO in a new vector system to evaluate the prokaryotic expression platform of the antimicrobial peptides. The fusion sequences were codon-optimized in three different combinations and expressed in E. coli.
WebWe have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the production of a variety of mammalian cytokines and growth factors as thioredoxin fusion proteins. Although m … WebBackground: To obtain thermal stable, soluble, biologically active hepatitis E virus recombinant antigen using thioredoxin fusion expression system. Methods: HEV ORF2 gene fragment (6964-7126 nt) was inserted into thioredoxin fusion expression vector pThioHisA. The recombinant plasmid was transformed into E. coli BL21 strain. After induction with …
WebMay 8, 2012 · Our new system was based on the co-expression of Avi-tagged target protein (as a thioredoxin fusion) and MBP–BirA, the schematic representations of which are shown in Supplementary Fig. 3. These two fusion proteins were well expressed and mainly found in the soluble portion when protein expression was induced at 30 °C (Fig. 2a, Lane 1–3 ...
WebAug 9, 2011 · Construction of thioredoxin gene fusion expression vectors. The two expression vectors constructed in this study, called pTX8048 and pTX8049, are derived from the high-copy number vector pNZ8048 harbouring the NICE system and a chloramphenicol resistance marker [].The high-copy number expression vector pTX8048 was constructed … pipe insulation casingWebNov 4, 2014 · Overexpression of the his 6-thioredoxin-GSL1 fusion protein in E. coli. The his 6-thioredoxin-GSL1 fusion protein was overexpressed in the E. coli strain BL21 (DE3) using the pET-32a vector. In comparison with thioredoxin alone (Figure 2A, lanes 5-7, right arrow), very little GSL1–thioredoxin fusion protein was expressed (Figure 2A, lanes 2–4), as … steph tan photographyWeb2T-T has a TEV-cleavable N-terminal His6-thioredoxin fusion tag. Thioredoxin can improve the solubility of your target protein by promoting correct disulfide bond formation. To … pipe insulation company near meWeb2T-T has a TEV-cleavable N-terminal His6-thioredoxin fusion tag. Thioredoxin can improve the solubility of your target protein by promoting correct disulfide bond formation. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use ... pipe insulation contractorWebMay 1, 1997 · This unit describes a gene fusion expression system that uses thioredoxin, the product of the Escherichia coli trxA gene, as the fusion partner. The inherent thermal … steph taxesWebAlthough thioredoxin fusion proteins can be readily purified by classical ion exchange chromatographic methods, we wanted to devise a convenient, generic, high-throughput affinity purification step for the thioredoxin fusion system. ... cloned in the expression vector, pALtrxA-781(3. LaVallie E.R. DiBlasio E.A. Kovacic S. Grant K.L. Schendel P ... steph taylor bristolWebDec 27, 2024 · Human enterokinase light chain (hEKL) cDNA sequence was designed with the help of codon optimization towards Escherichia coli codon preference and ribosome binding site design and artificially synthesized with a thioredoxin fusion tag at the N-terminal and a five his-tag peptide at the C-terminal. The synthetic hEKL gene was cloned into the … steph tan couture