Chromotek gfp-trap
WebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 …
Chromotek gfp-trap
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WebThere is a good alternative solution provided by NanoTag Biotechnologies for GFP pull downs, a GFP Selector resin, based on a high-affinity single-domain antibody (... WebApr 10, 2024 · Cell lysates were subjected to GFP-trap. The WCLs and GFP-trap products were immunoblotted for the indicated proteins and phospho-proteins. c, d, HEK293T cells expressing Ctrl or ILF3 shRNAs...
WebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg-Martinsried Germany phone: +49 89 124 148 80 fax: +49 89 124 148 811 ChromoTek Inc. 62-64 Enter Lane Islandia, NY 11749 USA phone: 631 501 1058 fax: 631 501 1060 … WebIam using the GFP Trap MA from chromotek for Ip of my protein. I followed everything as per the protocol, but was not successful. My protein was seen in the flowthrough, which …
WebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … WebThese reagents allow your GFP-fusions to be perfect candidates for immunoprecipitations, Co-IP, mass spectroscopy, enzyme activity measurements, and ChIP analysis. GFP-Trap Affinity Reagents are perfect for fast, clean, and efficient one-step immuno-purification of these fluorescent fusion proteins and their interacting factors.
The GFP-Trap is a ready-to-use reagent that consists of a GFP Nanobody conjugated to beads. Hence, it does not need the additional incubation step required when using Protein A/G beads and conventional antibodies for immunoprecipitation. Experimental and hands-on times are significantly reduced.
WebGFP Nanobody Magnetic Agarose, ChromoTek GFP-Trap® from Proteintech Group Inc This product is no longer available on Biocompare. Product Specs Item GFP Nanobody … simple warehouse managementWebThe ChromoTek GFP-Trap® is a ready to use affinity resin for immunoprecipitation (IP) of GFP-fusion proteins. GFP-Trap consists of an anti-GFP Nanobody/ VHH coupled to … simpleware安装包WebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ... simpleware破解版下载WebHello, you can melt Chromotek GFP-TRAP at 95*С and use magnet to remove magnetic agarose beads as you described without problem. Cite 3 Recommendations 21st Jan, 2024 Adam Thiesen Massachusetts... simpleware 破解版WebThe high binding rate of the ChromoTek GFP-Trap enables a considerable shorter processing time than conventional anti-GFP-antibodies. After washing, the immunocprecipitated protein fraction may be subject to Western blotting, mass spectrometry analysis, ChiP, iCLIP, RIP or enzyme activity assays. ray kennedy footballer wikiWebAug 27, 2024 · ChromoTek GFP-Trap ® is optimized for the immunoprecipitation of GFP-tagged proteins and their interacting factors. The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ V H H … ray kennedy - just for the moment 歌詞WebJun 8, 2024 · ChromoTek GFP-Trap enables a considerably shorter processing time than conventional anti-GFP-antibodies. For GFP-Trap, a 30-60 min incubation time at 4°C is … ray keplin-group.com